Flow perfusion rate modulates cell deposition onto scaffold substrate during cell seeding
نویسندگان
چکیده
منابع مشابه
A scaffold cell seeding method driven by surface acoustic waves.
Surface acoustic waves (SAW) have been employed to drive a particle suspension into a porous scaffold as a means for cell seeding. Straight, simple interdigital electrode structures were fabricated on lithium niobate to permit the generation of Rayleigh SAW radiation. Fluorescent microscopy was used to investigate the seeding process; the SAW-driven seeding process occurred in approximately 10s...
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Flow visualization using fluorescent microparticles and cell viability investigations are carried out to examine the mechanisms by which cells are seeded into scaffolds driven by surface acoustic waves. The former consists of observing both the external flow prior to the entry of the suspension into the scaffold and the internal flow within the scaffold pores. The latter involves micro-CT (comp...
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Tissue engineering has been developed as a new approach in regenerating tissues and organs using cells and scaffolds. An important element in successful tissue engineering in vitro is a suitable bioreactor system (Freed and Vunjak-Novakovic 1997). In general, some types of bioreactors have been used for cell culture on polymer scaffolds, namely spinner flasks, rotating vessels, and perfusion sy...
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In vitro studies on cancer cell lines still constitutes a major part of cancer research. It is very important that the researchers consider the importance of basic conditions of working with different cell lines. To the best of authors knowledge, this study is the first one to report the effect of seeding density on the growth curve of three human cancer cell lines. These cell lines include hum...
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In this study, we built a 3D perfusion system capable of applying oscillatory fluid shear stress on cells seeded in PLA scaffold. Subsequently, we studied responses of bone marrow cells to oscillatory perfusion. We first examined the responses of bone marrow cells from wild type (WT) mice and P2Y2 knockout (KO) mice to oscillatory perfusion. We found that oscillatory perfusion caused ERK1/2 pho...
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ژورنال
عنوان ژورنال: Biomechanics and Modeling in Mechanobiology
سال: 2017
ISSN: 1617-7959,1617-7940
DOI: 10.1007/s10237-017-0985-4